Discovering Antigen-specific Monoclonal Antibodies Using Single B Cell Screening

Single B cell screening is a powerful and efficient strategy for generating antigen-specific mAbs based on the direct amplification of the VH and VL regions encoding genes from single B cells. Notably, single B cell screening has various advantages that include maintaining the naïve VH/VL pairing, requiring relatively few cells, and the ability to discover antibodies against challenging targets.

Single B cell screening technologies have emerged and evolved rapidly over a decade. The process involves single B cell isolation, culture, sequencing, and cloning and the screening of selected single B cell antibodies. At the initial B cell screening stage, multiple platforms, including Berkely Lights Beacon®, fluorescence-activated cell sorting (FACS), and bead-based or FRET-based technologies, are applied. At Sino Biological, our team has established mature Beacon platform and flow cytometry platform for high throughput screening.

Download this application note to learn more about:

  • Workflow of Sino Biological's Single B-Cell Antibody Discovery Platform
  • Single B-Cell Screening using Multiple Technology Platforms
  • The Advantages and Disadvantages of Single B cell Screening Platforms
  • Application Notes and Case Studies



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